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KMID : 0380619830150020170
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Korean Journal of Food Science and Technology
1983 Volume.15 No. 2 p.170 ~ p.176
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Analysis of Cellular Components of Starch - Utilizing Yeast Sporobolomyces holsaticus
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신동화/Shin, Dong Hwa
서기봉/구영조/박완수/Suh, Kee Bong/Koo, Young Jo/Park, Wan Soo
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Abstract
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Starchy single cell protein produced by a starch-utilizing yeast, Sporobolomyces holsaticus FRI Y-5 was analyzed for its composition such as intracellular protein, amino acids, fatty acids, minerals, vitamins and pigments. It was shown that it contained 33.08% of total carbohydrate, 45.63% of crude protein, 20.01% of crude lipid, 3.24% of ash and 4.46% of pigment. Whole cell extracted by cold and hot NaOH method contained 40.89% of soluble protein and the estimated nucleic acid content from crude and soluble protein contents was about 7.6%. The sulphur-containing amino acids, threonine, isoleucine and valine were analyzed to be the limiting amino acids in the starchy SCP, and the protein score was calculated as 89.4. It was shown from its fatty acid analysis that it contained 6.5% of C_(16:0), 2.4% of C_(18:0), 81.9% of C_(18:1), 3.2% of C_(18:2), and 6.0% of C_(18:3). Also it was observed that it contained, per 100 g of dry cell, 365.33㎎ of Mg and 282.75㎎ of K more than Fe and Ca. The content of Vit. B₂ was 3.7㎎ per 100 g of dry cell, but niacin was not detected under this experimental condition. The UV-visible scanning result of pigment extract showed that the yeast contained carotenoid and unknown pigments.
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